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CAS RN: 127274-91-3 | Product Number: D6263
DiD Solution [for Cell Membrane Staining]
Purity:
Synonyms:
- 2-[5-(3,3-Dimethyl-1-octadecylindolin-2-ylidene)penta-1,3-dien-1-yl]-3,3-dimethyl-1-octadecyl-3H-indol-1-ium Perchlorate Solution
Product Documents:
Size | Unit Price | Belgium | Japan* | Quantity |
---|---|---|---|---|
1ML |
€226.00
|
Contact Us | 14 |
|
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
Supplemental Product Information:
Product Information
- Concentration : 1 mM
- Excitation / Emission : 657 nm / 678 nm
Description
- DiD is a lipophilic carbocyanine dye used to label cell membranes. It emits strong fluorescence when its long hydrocarbon chains are incorporated into a lipid bilayer. This product is a ready-to-use solution of DiD which can be used as a tracer in cell fusion, cell adhesion and chemotaxis.
Storage
- Store at room temperature and protect from light.
Directions for Use: Staining live cells
-
- Dilute DiD solution to 5 μM with culture medium.
- Remove medium from cells and replace with 5 μM DiD-containing medium.
- Incubate cells at 37 °C for 10 minutes.
- Remove medium and wash cells 3 times with PBS.
- Observe stained cell membranes via a fluorescence microscope.
Directions for Use: Staining fixed cells
-
- Remove culture medium and wash cells twice with PBS.
- Add 4% paraformaldehyde to cells and incubate at room temperature for 10 minutes.
- Dilute DiD solution to 5 μM with PBS.
- Remove 4% paraformaldehyde and add 5 μM DiD-containing PBS to cells.
- Incubate cells at 37 °C for 10 minutes.
- Remove medium and wash cells 3 times with PBS.
- Observe stained cell membranes via a fluorescence microscope.
Product Number | D6263 |
Molecular Formula / Molecular Weight | C61H99ClN2O4 = 959.92 |
Physical State (20 deg.C) | Liquid |
Storage Temperature | Room Temperature (Recommended in a cool and dark place, <15°C) |
CAS RN | 127274-91-3 |
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