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CAS RN: 28718-90-3 | Product Number: A2412
DAPI 2HCl [for Biochemical Research]
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![DAPI 2HCl [for Biochemical Research]](/_ui/responsive/theme-tci/images/missing_product_zoom.jpg "DAPI 2HCl [for Biochemical Research]")
Purity: >90.0%(HPLC)
Synonyms:
- 4',6-Diamidino-2-phenylindole Dihydrochloride
- 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide Dihydrochloride
- 6-Amidino-2-(4-amidinophenyl)indole Dihydrochloride
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
|---|---|---|---|---|
| 5MG |
44,00 €
|
4 | 9 |
|
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
| Product Number | A2412 |
| Purity / Analysis Method | >90.0%(HPLC) |
| Molecular Formula / Molecular Weight | C__1__6H__1__5N__5·2HCl = 350.25 |
| Physical State (20 deg.C) | Solid |
| Storage Temperature | Frozen (<0°C) |
| Condition to Avoid | Light Sensitive,Heat Sensitive |
| CAS RN | 28718-90-3 |
| Reaxys Registry Number | 4894417 |
| PubChem Substance ID | 135727109 |
| MDL Number | MFCD00012681 |
Specifications
| Appearance | Light yellow to Yellow to Orange powder to crystal |
| Purity(HPLC) | min. 90.0 area% |
| DNA staining test | to pass test |
Properties (reference)
GHS
Related Laws:
| EC Number | 249-186-7 |
Transport Information:
| HS Number | 2933998090 |
Application
Fluorescence Staining of Cell Nucleus
DAPI is an organic dye which binds strongly to DNA. DAPI emits a blue fluorescence (Maximum fluorescent wavelength; 461 nm) by the irradiation of UV light (Maximum excitation wavelength; 358 nm).
HeLa cell nucleus stained with DAPI·2HCl [A2412] has been observed by fluorescent microscopy.
HeLa cell nucleus stained with DAPI·2HCl [A2412] has been observed by fluorescent microscopy.
HeLa cells were fixed with 4% paraformaldehyde, and permerabilized with 0.2% Triton X-100.
HeLa cells nuclei were stained with 1 µg/mL DAPI [A2412] solution.
HeLa cells nuclei were stained with 1 µg/mL DAPI [A2412] solution.
References
- Labeling Nuclear DNA Using DAPI
PubMed Literature
Articles/Brochures
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DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]
| Product # | D5888 |
| CAS RN | 28718-90-3 |
| Purity / Analysis Method: |