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Indicator Nucleoside of DNA Methylation
No.148(August 2012)
Biological consequences of DNA methylation are indicators of epigenetic effects in plants and animals. 2’-Deoxy-5-methylcytidine, formed by the enzymatic methylation of cytosine residues in CpG (Cytosine-phosphate-Guanine) sites, is a minor component found in most eukaryotic DNA which plays a central role in the regulation of gene expression. Recently, methylation occurring by methyl radicals generated by tumor promoters has been reported.1) Oxidation of 2’-deoxy-5-methylcytidine has been of interest in connection with the damage of DNA.2) In the detection of DNA methylation levels in cells, the DNA is hydrolyzed to nucleosides and 2’-deoxy-5-methylcytidine is analyzed by HPLC and LC-MS.3)
References
- 1)Cytosine C-5 methylation by methyl radicals generated by tumor promoters
- 2)Oxidation studies of 2’-deoxy-5-methylcytidine
- 3)Analyzed of 2’-deoxy-5-methylcytidine in cells
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