N,N'-Diisopropyl-O-(4-nitrobenzyl)isourea [for HPLC Labeling]

The compound 1 easily reacts with a carboxyl group to form the corresponding ester without using a catalyst or an activating agent. The resultant ester is stable and can reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by measuring at 254 nm, an absorption wavelength that is generally used for UV detection.

Application examples:
[Fatty acids] ¹⁾
Dissolve 5 mg of a sample in CH₂Cl₂ (1 mL), and add the labeling reagent 1 (20 mg) in CH₂Cl₂ (2 mL). Close the cap of the reaction vessel and incubate the solution at 80 °C for 2 h. Cool the resultant solution to room temperature and use it as an HPLC sample.

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )