“Non-Fucosyl G2 N-Glycan” Expected as a High-Functional Glycoform for Therapeutic Antibody

G2-peptide (1) is practically available as a structure-defined glycan donor in the “enzymatic N-glycan remodeling technology” to convert glycosylation of glycoprotein from heterogeneous non-core fucosylated biantennary N-glycans to homogeneous G2 N-glycan. It has been reported that antibodies possessing "non-fucosyl G2 glycoform” show functional improvements in physiological phenomena such as antibody-dependent cellular cytotoxicity (ADCC) activity, complement-dependent cytotoxicity (CDC) activity, and anti-inflammatory effect by the US National Institutes of Health (NIH) and the JP National Institute of Health Sciences (NIHS) and other institutes. Thus, "non-fucosyl G2 N-glycan” has been an attractive glycan structure in biopharmaceutical fields in recent years.^1-4) Please contact us for mass production of 1. In addition, G2 glycan (2), G2 2AB (500pmol/vial) (3) and nonasaccharide-β-pNP (4) are also available as reagents for comparative analyses, enzyme substrates and so on.

References

• 1) Crystallizable Fragment Glycoengineering for Therapeutic Antibodies Development
  • W. Li, Z. Zhu, W. Chen, Y. Feng, D. S. Dimitrov, Front. Immunol. 2017, 8, 1554.
• 2) Assessing the Heterogeneity of the Fc-Glycan of a Therapeutic Antibody Using an engineered FcγReceptor IIIa-Immobilized Column
  • M. Kiyoshi, J. M. M. Caaveiro, M. Tada, H. Tamura, T. Tanaka, Y. Terao, K. Morante, A. Harazono, N. Hashii, H. Shibata, D. Kuroda, S. Nagatoishi, S. Oe, T. Ide, K. Tsumoto, A. Ishii-Watabe, Sci. Rep. 2018, 8, 3955.
• 3) Conformational effects of N-glycan core fucosylation of immunoglobulin G Fc region on its interaction with Fcγ receptor IIIa
  • Y. Sakae, T. Satoh, H. Yagi, S. Yanaka, T. Yamaguchi, Y. Isoda, S. Iida, Y. Okamoto, K. Kato, Sci Rep. 2017, 7, 13780.
• 4) Anti-inflammatory activity of galactosylated, nonfucosylated intravenous immunoglobulin
  • Y. Mimura, Trends Glycosci. Glycotech. 2022, 25, A11.

Related Compounds

A1651

endo-β-N-Acetylglucosaminidase (=Endo-M) Recombinant: from Mucor hiemalis expressed in Candida boidinii [Purity: single band by SDS-PAGE(85KDa)]

G0365

Glycosynthase (Endo-M-N175Q) Recombinant: from Mucor hiemalis expressed in Escherichia coli (100m units/vial)

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