Peroxidase Detection

No.164(January 2015)

S0887: Sodium Perborate Tetrahydrate (1)

Chemiluminescence has contributed to providing high sensitivity for enzyme-linked immunoassays.^1,2) Thorpe et al. referred that, when peroxidase is used for immunoassay as a labeled enzyme, hydrogen peroxide can be replaced by oxidants such as perborate.²⁾ Compound 1 is the sodium salt of perborate. In their study,³⁾ they used the sodium salt to replace hydrogen peroxide. 1 has already been employed for recent improvements of immunoassay.^4,5)

On the other hand, interestingly, 1 has already been used as a substrate of catalase in the late 1940’s.⁶⁾

References

1)Chemiluminescent enzyme immunoassay, a review
- A. Tsuji, M. Maeda, H. Arakawa, Anal. Sci. 1989, 5, 497.
2)Enhanced chemiluminescence enzyme immunoassay
- L. J. Kricka, G. H. G. Thorpe, R. A. W. Stott, Pure Appl. Chem. 1987, 59, 651.
3)Phenols as enhancers of the chemiluminescent horseradish peroxidase–luminol–hydrogen peroxide reaction: Application in luminescence-monitored enzyme immunoassays
- G. H. G. Thorpe, L. J. Kricka, S. B. Moseley, T. P. Whltehead, Clin. Chem. 1985, 31, 1335.
4)Inhibition of horseradish peroxidase (HRP) by a nonhydrophobic component of urine: A caution for immunoassays
- K. L. Campbell, M. Lopresti, W. Lukas, Open Clin. Chem. J. 2014, 7, 1.
5)Development of a highly sensitive chemiluminescence enzyme immunoassay using enhanced luminol as substrate
- X. Tao, W. Wang, Z. Wang, X. Cao, J. Zhu, L. Niu, X. Wu, H. Jiang, J. Shen, Luminescence 2014, 29, 301.
6)Perborate as substrate in a new assay of catalase
- R. N. Feinstein, J. Biol. Chem. 1949, 180, 1197.

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