适用于蛋白质处理的非洗涤剂型磺基三甲铵乙内酯(NDSB)

蛋白质提取和分析中的溶解过程需要使用到如SDS和LDS，变性剂尿素和胍这样的表面活性剂。然而，由于表面活性剂与蛋白质之间的相互作用很强，蛋白质重折叠和纯化在某些情况下可能比较困难。
含有阳离子和阴离子的两性小分子化合物，如3-[(2-hydroxyethyl)dimethylammonio]propane-1-sulfonate (NDSB 211), 3-(1-pyridinio)propanesulfonate (NDSB 201)和3-(4-tert-butyl-1-pyridinio)propanesulfonate (NDSB 256-4T)被称为非洗涤剂型磺基三甲铵乙内酯(NDSB)，其与一般表面活性剂相比具有不同性质。因为它具有较小的疏水端并能温和地溶解蛋白质，NDSB不会形成胶束。而且已有报道称NDSB能够有效地预防蛋白质在热、酸条件下的变性，抑制蛋白质聚集，加速蛋白质的重折叠以及提高膜蛋白质提取物的质量。

关键词

H1399: 3-[(2-Hydroxyethyl)dimethylammonio]propane-1-sulfonate (NDSB 211)
S0813: 3-(1-Pyridinio)propanesulfonate (NDSB 201)
B4030: 3-(4-tert-Butyl-1-pyridinio)propanesulfonate (NDSB 256-4T)

文献

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适用于蛋白质处理的非洗涤剂型磺基三甲铵乙内酯(NDSB)

关键词

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