一种用于抗氧化能力检测的标准抗氧化剂——水溶性维生素E

食物中的许多成分，如多酚类物质等，具有清除自由基的抗氧化活性，且该活性可以通过检测其体外电位进行评估^1,2)。根据不同的原理，抗氧化能力检测可分为两种类型：基于氢原子转移(HAT)反应和基于电子转移(ET)反应。基于HAT的检测包括氧自由基吸收(ORAC)法^3,4)和总自由基清除抗氧化能力(TRAP)法⁵⁾，ORAC法是对荧光素的荧光强度进行分析，而荧光素由AAPH(2,2'-azobis(2-methylpropionamidine) dihydrochloride)产生的过氧自由基分解得到。TRAP法是对被类似的过氧自由基氧化的鲁米诺试剂的荧光强度进行分析。基于ET的检测包括DPPH法⁶⁾和TEAC法⁷⁾等，它们分别对DPPH(1,1-diphenyl-2-picrylhydrazyl)和ABTS(2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid ammonium salt))进行色度分析。

水溶性维生素E诞生于于1974年，是一种维生素E衍生的水溶性抗氧化剂⁸⁾。作为一种标准抗氧化剂，它已被用于这些抗氧化能力的检测中。

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一种用于抗氧化能力检测的标准抗氧化剂——水溶性维生素E

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