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CAS RN: | 产品编码: T4032

Clearing Reagent iCBiofilm-H2 [for Biofilm]


纯度/分析方法:
别名:
  • 组织透明化试剂iCBiofilm-H2 [生物膜用]
产品文档:
8ML
¥260.00
1   从上海仓库发货 12  
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补充产品信息:

Product Information

  • Size : 8 mL

Description

  • Biofilms are highly organized structures of microbial communities composed of a matrix of polysaccharides and other substances secreted by microbes and/or aggregates of microbial cells. Microbial cells become resistant to antimicrobial drugs and host defense systems when they form biofilms.
    In order to observe the structures of biofilms with a thickness of more than several tens of micrometers, Clearing Reagent iCBiofilm-H2 has been developed. iCBioflim-H2 is supplied as a stock solution. Before adding to biofilm, iCBiofilm-H2 has to be diluted with deionized water to appropriate concentration (undiluted iCBiofilm-H2 may work well). By adding iCBiofilm-H2 solution to a biofilm, the biofilm becomes transparent within several seconds and easy-to-observe by light microscopy such as confocal laser scanning microscopy.
    *This product was developed by Associate Prof. Sugimoto, the Jikei University School of Medicine.

Storage

  • Stored at room temperature and used within 6 months.

Direction for Use

    1. Prepare a biofilm in a glass bottomed culture dish (35mm diam.). Culture conditions (including medium, temperature, time, etc.) all play roles in biofilm formation; the conditions specific to your microbe of interest must be determined separately.
    2. After removing planktonic cells and culture medium, add 1% glutaraldehyde solution in PBS and fix at room temperature for 30 minutes. To preserve immunogenicity for immunofluorescence applications, we recommend substituting 4% paraformaldehyde in PBS.
    3. Remove these glutaraldehyde solution and wash three times with PBS or distilled water.
    4. Stain using appropriate fluorescence reagents.
    5. Dilute iCBiofilm-H2 (Product No. T4032) with deionized water to an appropriate concentration.
      Recommendation ratio; iCBiofilm-H2 : water = 2 : 1 or 1 : 1
      If you need the best performance of iCBioflim-H2 for a target biofilm, please consider to examine different concentrations of iCBiofilm-H2.
    6. Remove staining reagents and add diluted or undiluted iCBiofilm-H2 solution (about 500 µL – 1 mL).
    7. Observe the biofilm by optical microscopy.

Related Products

  • T4031 Clearing Reagent iCBiofilm-H1 [for Biofilm]
  • I1275 Clearing Reagent iCBiofilm-D [for Biofilm]

产品编码 T4032
外观与形状(20°C) 液体
储存温度 室温 (15°C以下阴凉干燥处)
技术规格
Appearance Colorless to Almost colorlessclear liquid
Functionality test to pass test
Refractive index 1.4550 to 1.4650
物性(参考值)
GHS
相关法规
运输信息
监管条件代码(*)
应用
Clearing of biofilm

Reference


应用
Microscopic observation of biofilm

References


产品文档 (部分产品的分析图谱无法提供,敬请谅解。)
化学品安全说明书(SDS)
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