轻松扫码查看产品文档 | TCIMAIL No.197 已上新 | TCI试剂——品质可靠,值得信赖
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CAS RN: | 产品编码: T4032
Clearing Reagent iCBiofilm-H2 [for Biofilm]
* 点击“查询”可查看预计发货日期,仅供参考。
* 无具体发货日期的情况,如:显示“8个工作日后发货”,将在您订购日起的8个工作日后发货。
* 我们将以最优方式从上海/天津两大仓库发货。国内库存不足,需两周左右向日本总部调货。
* 对于可分装产品,11:30前的订单,当天发货;11:30后的订单,隔天发货。
* 如需大包装,请点击“大包装询价”按钮(对于某些产品我们无法提供大包装)。
* TCI会经常复审储藏条件以对其进行优化,请以在线目录为准,敬请留意。
* 更多信息,请联系营业部:021-67121386 / Sales-CN@TCIchemicals.com 。任何货期、规格或包装方面的需求,请联系我们 。
* 无具体发货日期的情况,如:显示“8个工作日后发货”,将在您订购日起的8个工作日后发货。
* 我们将以最优方式从上海/天津两大仓库发货。国内库存不足,需两周左右向日本总部调货。
* 对于可分装产品,11:30前的订单,当天发货;11:30后的订单,隔天发货。
* 如需大包装,请点击“大包装询价”按钮(对于某些产品我们无法提供大包装)。
* TCI会经常复审储藏条件以对其进行优化,请以在线目录为准,敬请留意。
* 更多信息,请联系营业部:021-67121386 / Sales-CN@TCIchemicals.com 。任何货期、规格或包装方面的需求,请联系我们 。
补充产品信息:
Product Information
- Size : 8 mL
Description
- Biofilms are highly organized structures of microbial communities composed of a matrix of polysaccharides and other substances secreted by microbes and/or aggregates of microbial cells. Microbial cells become resistant to antimicrobial drugs and host defense systems when they form biofilms.
In order to observe the structures of biofilms with a thickness of more than several tens of micrometers, Clearing Reagent iCBiofilm-H2 has been developed. iCBioflim-H2 is supplied as a stock solution. Before adding to biofilm, iCBiofilm-H2 has to be diluted with deionized water to appropriate concentration (undiluted iCBiofilm-H2 may work well). By adding iCBiofilm-H2 solution to a biofilm, the biofilm becomes transparent within several seconds and easy-to-observe by light microscopy such as confocal laser scanning microscopy.
*This product was developed by Associate Prof. Sugimoto, the Jikei University School of Medicine.
Storage
- Stored at room temperature and used within 6 months.
Direction for Use
-
- Prepare a biofilm in a glass bottomed culture dish (35mm diam.). Culture conditions (including medium, temperature, time, etc.) all play roles in biofilm formation; the conditions specific to your microbe of interest must be determined separately.
- After removing planktonic cells and culture medium, add 1% glutaraldehyde solution in PBS and fix at room temperature for 30 minutes. To preserve immunogenicity for immunofluorescence applications, we recommend substituting 4% paraformaldehyde in PBS.
- Remove these glutaraldehyde solution and wash three times with PBS or distilled water.
- Stain using appropriate fluorescence reagents.
- Dilute iCBiofilm-H2 (Product No. T4032) with deionized water to an appropriate concentration.
Recommendation ratio; iCBiofilm-H2 : water = 2 : 1 or 1 : 1
If you need the best performance of iCBioflim-H2 for a target biofilm, please consider to examine different concentrations of iCBiofilm-H2. - Remove staining reagents and add diluted or undiluted iCBiofilm-H2 solution (about 500 µL – 1 mL).
- Observe the biofilm by optical microscopy.
Related Products
- T4031 Clearing Reagent iCBiofilm-H1 [for Biofilm]
- I1275 Clearing Reagent iCBiofilm-D [for Biofilm]
产品编码 | T4032 |
外观与形状(20°C) | 液体 |
储存温度 | 室温 (15°C以下阴凉干燥处) |
技术规格
Appearance | Colorless to Almost colorlessclear liquid |
Functionality test | to pass test |
Refractive index | 1.4550 to 1.4650 |
物性(参考值)
GHS
相关法规
运输信息
监管条件代码(*) |
应用
Clearing of biofilm
Reference
- Instantaneous Clearing of Biofilm (iCBiofilm): an optical approach to revisit bacterial and fungal biofilm imaging
应用
Microscopic observation of biofilm
References
- Light and Electron Microscopic Studies of Microorganisms Growing in Rotating Biological Contactor Biofilms
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