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CAS RN: | 产品编码: T3885

Streptavidin R-PE Conjugate


纯度/分析方法:
别名:
  • 链霉亲和素R-PE结合物
  • SA-R-PE
产品文档:
1VIAL
¥465.00
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补充产品信息:

Product Information

Volume/Quantity : 0.1 mg
Product Form : Purified streptavidin, R-Phycoerythrin (R-PE) conjugated.
Conjugation : Purified streptavidin was conjugated to R-PE (Ex:496 nm, Em:575 nm)
Buffer : Phosphate buffered-saline(-) (PBS(-))
Preservative : 0.05% Sodium azide
Specificity : Biotin

Application

Immunofluorescence (1:200). Flow cytometry (1:1000). FLISA (1:400). Western blotting (1:1000).


Storage

Store at 4 ºC. Do Not Freeze. This product is photosensitive and should be protected from light.


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产品编码 T3885
外观与形状(20°C) 液体
储存温度 冷藏 (0-10°C)
应避免的情况 光,加热
技术规格
Appearance Light red to Red clear liquid to cloudy liquid
Specific Activity to pass test(FLISA)
物性(参考值)
GHS
相关法规
新化学物质备案回执号 B1A232216177
运输信息
监管条件代码(*) Q类 (糖类,生物碱类,抗生素类,激素类)
应用
Immunofluorescence Staining

The HeLa cells were fixed with 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100. Then, the specimen was blocked in BSA/PBS blocking buffer. Subsequently, the specimen was incubated with properly diluted primary antibody (Mouse Anti α-Tubulin IgG and Rabbit Anti AIF* IgG). And, after washing, was further incubated with biotin-conjugated secondary antibody (Goat Anti-Mouse IgG Biotin Conjugate [G0387]). Finally, it was rinsed with PBS and stained with each fluorescent-probe**.*Apoptosis Inducing Factor (AIF) normally localizes in the inner mitochondrial membrane.
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL (shown in red
   Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL (shown in green
   Hoechst 33258 [H1343] 1 µg/mL(shown in blue


应用
Flow Cytometry of Stained Cells

The HeLa cells were incubated with Mouse Anti CD9 Antibody (red line) or Mouse IgG2aκ isotype control (black line) at 1μg/mL. Then both were incubated with Goat Anti-Mouse IgG Biotin Conjugate [G0387] at 1 µg/mLas a secondary antibody, followed by staining with Streptavidin R-PE Conjugate [T3885] at 1 µg/mL.

References


TCIMail
产品文档 (部分产品的分析图谱无法提供,敬请谅解。)
化学品安全说明书(SDS)
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技术规格
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其他文件

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