盲盒好礼,精彩继续 | 轻松扫码查看产品文档 | TCIMAIL No.198 已上新 | TCI试剂——品质可靠,值得信赖
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CAS RN: | 产品编码: T3885
Streptavidin R-PE Conjugate
* 点击“查询”可查看预计发货日期,仅供参考。
* 无具体发货日期的情况,如:显示“8个工作日后发货”,将在您订购日起的8个工作日后发货。
* 我们将以最优方式从上海/天津两大仓库发货。国内库存不足,需两周左右向日本总部调货。
* 对于可分装产品,11:30前的订单,当天发货;11:30后的订单,隔天发货。
* 如需大包装,请点击“大包装询价”按钮(对于某些产品我们无法提供大包装)。
* TCI会经常复审储藏条件以对其进行优化,请以在线目录为准,敬请留意。
* 更多信息,请联系营业部:021-67121386 / Sales-CN@TCIchemicals.com 。任何货期、规格或包装方面的需求,请联系我们 。
* 无具体发货日期的情况,如:显示“8个工作日后发货”,将在您订购日起的8个工作日后发货。
* 我们将以最优方式从上海/天津两大仓库发货。国内库存不足,需两周左右向日本总部调货。
* 对于可分装产品,11:30前的订单,当天发货;11:30后的订单,隔天发货。
* 如需大包装,请点击“大包装询价”按钮(对于某些产品我们无法提供大包装)。
* TCI会经常复审储藏条件以对其进行优化,请以在线目录为准,敬请留意。
* 更多信息,请联系营业部:021-67121386 / Sales-CN@TCIchemicals.com 。任何货期、规格或包装方面的需求,请联系我们 。
补充产品信息:
Product Information
Volume/Quantity : 0.1 mgProduct Form : Purified streptavidin, R-Phycoerythrin (R-PE) conjugated.
Conjugation : Purified streptavidin was conjugated to R-PE (Ex:496 nm, Em:575 nm)
Buffer : Phosphate buffered-saline(-) (PBS(-))
Preservative : 0.05% Sodium azide
Specificity : Biotin
Application
Immunofluorescence (1:200). Flow cytometry (1:1000). FLISA (1:400). Western blotting (1:1000).
Storage
Store at 4 ºC. Do Not Freeze. This product is photosensitive and should be protected from light.
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产品编码 | T3885 |
外观与形状(20°C) | 液体 |
储存温度 | 冷藏 (0-10°C) |
应避免的情况 | 光,加热 |
技术规格
Appearance | Light red to Red clear liquid to cloudy liquid |
Specific Activity | to pass test(FLISA) |
物性(参考值)
GHS
相关法规
新化学物质备案回执号 | B1A232216177 |
运输信息
监管条件代码(*) | Q类 (糖类,生物碱类,抗生素类,激素类) |
应用
Immunofluorescence Staining
The HeLa cells were fixed with 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100. Then, the specimen was blocked in BSA/PBS blocking buffer. Subsequently, the specimen was incubated with properly diluted primary antibody (Mouse Anti α-Tubulin IgG and Rabbit Anti AIF* IgG). And, after washing, was further incubated with biotin-conjugated secondary antibody (Goat Anti-Mouse IgG Biotin Conjugate [G0387]). Finally, it was rinsed with PBS and stained with each fluorescent-probe**.*Apoptosis Inducing Factor (AIF) normally localizes in the inner mitochondrial membrane.
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL (shown in red)
Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL (shown in green)
Hoechst 33258 [H1343] 1 µg/mL(shown in blue)
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL (shown in red)
Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL (shown in green)
Hoechst 33258 [H1343] 1 µg/mL(shown in blue)
应用
Flow Cytometry of Stained Cells
The HeLa cells were incubated with Mouse Anti CD9 Antibody (red line) or Mouse IgG2aκ isotype control (black line) at 1μg/mL. Then both were incubated with Goat Anti-Mouse IgG Biotin Conjugate [G0387] at 1 µg/mLas a secondary antibody, followed by staining with Streptavidin R-PE Conjugate [T3885] at 1 µg/mL.
References
- Guidelines for the use of flow cytometry and cell sorting in immunological studies
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