轻松扫码查看产品文档 | TCIMAIL No.197 已上新 | TCI试剂——品质可靠,值得信赖
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CAS RN: | 产品编码: R0243
rEGCase II assisted by Activator II
纯度/分析方法:
别名:
- 激活剂II辅助型rEGCase II
- 激活剂II辅助型重组神经节苷脂内切糖苷酶
- Recombinant Endoglycoceramidase II assisted by Activator II
- Recombinant - Rhodococcus sp. EGCase II expressed in Rhodococcus erythropolis
- Recombinant Endoglycoceramidase II with Activator II
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* TCI会经常复审储藏条件以对其进行优化,请以在线目录为准,敬请留意。
* 更多信息,请联系营业部:021-67121386 / Sales-CN@TCIchemicals.com 。任何货期、规格或包装方面的需求,请联系我们 。
* 无具体发货日期的情况,如:显示“8个工作日后发货”,将在您订购日起的8个工作日后发货。
* 我们将以最优方式从上海/天津两大仓库发货。国内库存不足,需两周左右向日本总部调货。
* 对于可分装产品,11:30前的订单,当天发货;11:30后的订单,隔天发货。
* 如需大包装,请点击“大包装询价”按钮(对于某些产品我们无法提供大包装)。
* TCI会经常复审储藏条件以对其进行优化,请以在线目录为准,敬请留意。
* 更多信息,请联系营业部:021-67121386 / Sales-CN@TCIchemicals.com 。任何货期、规格或包装方面的需求,请联系我们 。
补充产品信息:
Product Information
- Quantity : 100 mU per vial (1 mU/μL) and 50 nmol Activator II per vial (0.5 nmol/μL)
- Product Form : Purified recombinant EGCase II and Activator II, Liquid
- Storage buffer : 20 mM sodium phosphate, 50 mM NaCl buffer, pH 7.0
- Preservative : None
- Stabilizer : None
Description
- Endoglycoceramidase II (EGCase II) is a glycolipid-specific hydrolase that cleaves the glycosidic linkage between oligosaccharide and ceramide of various glycosphingolipids (GSLs) under a detergent-containing condition. On the other hand, Activator II is capable of inducing activity of EGCase without any detergent reagents. This product is provided as a mixture-form of EGCase II and Activator II. By using this product, cell-surface glycosphingolipids of living cells are hydrolyzed without cell disruption caused by addition of detergent. The EGCase II gene is derived from Rhodococcus sp. and its recombinant protein is expressed in Rhodococcus erythropolis.
Unit Definition
- One unit (U) of EGCase II is defined as the amount required to hydrolyze 1 μmol of ganglioside GM1 per minute at 37°C under the condition of pH5.5.
Assay Conditions
- Reaction conditions: 50 mM sodium acetate, pH 5.5
The reaction mixture includes the enzyme and 100 nmol of GM1 in 50 μL (without detergent). The mixture is incubated at 37°C for 15 minutes. Quantification of reducing end of released oligosaccharides is measured by the modified Somogyi-Nelson method (see the following reference). - [Reference]
Determination of Glucose by a Modification of Somogyi-Nelson Method
C. Hatanaka, Y. Kobara, Agric. Biol. Chem. 1980, 44, 2943.
- Regarding hydrolysis of cellular GSLs, see the reference (Methods Mol. Biol.) below.
- [Reference]
Assays and Utilization of Enzymes Involved in Glycolipid Metabolism in Bacteria and Fungi
M. Ito, Y. Ishibashi, T. Watanabe, J. Iwaki, T. Kurita, N. Okino, Methods Mol. Biol. 2023, 2613, 229.
Storage
- Store at -20°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 4°C and use it within a few weeks.
Related Products
- G0483 Ganglioside GM1
- P1775 TRITON™ X-100
- Other oligosaccharide-releasing enzymes
- [Endo-type glycoceramidase]
- R0240 rEGCase I
- R0241 rEGCase I assisted by Activator II
- R0242 rEGCase II
- [Endo-type N-glycanase (ENGase)]
- A1651 Endo-M (for hydrolysis of non core-fucosylated biantennary N-glycan)
- E1339 Endo-M-W251N (for hydrolysis of core-fucosylated biantennary N-glycan)
- G0365 Glycosynthase (for transglycosylation of non core-fucosylated biantennary N-glycan)
- [Endo-type O-glycanase]
- A1844 Endo-α (for hydrolysis of core1 O-glycan epitope known as T/TF antigen)
- G0622 O-Glycosidase for core1 (for hydrolysis of sialylated core1 O-glycan epitope)
- [Endo-type glycosaminoglycanase]
- K0069 Keratanase II (for hydrolysis of Keratan sulfate)
产品编码 | R0243 |
外观与形状(20°C) | 液体 |
储存温度 | 冷冻 (-20°C) |
应避免的情况 | 加热 |
技术规格
Appearance | Colorless to Almost colorlessclear liquid |
Titer | min. 100 mU/Vial |
物性(参考值)
GHS
相关法规
新化学物质备案回执号 | B1A232224108 |
运输信息
监管条件代码(*) |
应用
Endoglycoceramidase
References
- Purification and characterization of glycosphingolipid-specific endoglycosidases (endoglycoceramidases) from a mutant strain of Rhodococcus sp. Evidence for three molecular species of endoglycoceramidase with different specificities
- Preparation and characterization of EGCase I, applicable to the comprehensive analysis of GSLs, using a rhodococcal expression system
文章/手册
TCIMail
[TCIMAIL No.185] Endoglycoceramidase (EGCase): Glycosphingolipid-hydrolyzing Enzyme[Glycoscience Products] Endoglycoceramidase ― Hydrolytic Enzymes Specific to Glycosphingolipids
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