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CAS RN: | 产品编码: R0243

rEGCase II assisted by Activator II


纯度/分析方法:
别名:
  • 激活剂II辅助型rEGCase II
  • 激活剂II辅助型重组神经节苷脂内切糖苷酶
  • Recombinant Endoglycoceramidase II assisted by Activator II
  • Recombinant - Rhodococcus sp. EGCase II expressed in Rhodococcus erythropolis
  • Recombinant Endoglycoceramidase II with Activator II
产品文档:
1VIAL
¥3,000.00
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补充产品信息:

Product Information

  • Quantity : 100 mU per vial (1 mU/μL) and 50 nmol Activator II per vial (0.5 nmol/μL)
  • Product Form : Purified recombinant EGCase II and Activator II, Liquid
  • Storage buffer : 20 mM sodium phosphate, 50 mM NaCl buffer, pH 7.0
  • Preservative : None
  • Stabilizer : None

Description

  • Endoglycoceramidase II (EGCase II) is a glycolipid-specific hydrolase that cleaves the glycosidic linkage between oligosaccharide and ceramide of various glycosphingolipids (GSLs) under a detergent-containing condition. On the other hand, Activator II is capable of inducing activity of EGCase without any detergent reagents. This product is provided as a mixture-form of EGCase II and Activator II. By using this product, cell-surface glycosphingolipids of living cells are hydrolyzed without cell disruption caused by addition of detergent. The EGCase II gene is derived from Rhodococcus sp. and its recombinant protein is expressed in Rhodococcus erythropolis.

Unit Definition

  • One unit (U) of EGCase II is defined as the amount required to hydrolyze 1 μmol of ganglioside GM1 per minute at 37°C under the condition of pH5.5.

Assay Conditions

Storage

  • Store at -20°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 4°C and use it within a few weeks.

Related Products

  • G0483 Ganglioside GM1
  • P1775 TRITON™ X-100

  • Other oligosaccharide-releasing enzymes
  • [Endo-type glycoceramidase]
  • R0240 rEGCase I
  • R0241 rEGCase I assisted by Activator II
  • R0242 rEGCase II

  • [Endo-type N-glycanase (ENGase)]
  • A1651 Endo-M (for hydrolysis of non core-fucosylated biantennary N-glycan)
  • E1339 Endo-M-W251N (for hydrolysis of core-fucosylated biantennary N-glycan)
  • G0365 Glycosynthase (for transglycosylation of non core-fucosylated biantennary N-glycan)

  • [Endo-type O-glycanase]
  • A1844 Endo-α (for hydrolysis of core1 O-glycan epitope known as T/TF antigen)
  • G0622 O-Glycosidase for core1 (for hydrolysis of sialylated core1 O-glycan epitope)

  • [Endo-type glycosaminoglycanase]
  • K0069 Keratanase II (for hydrolysis of Keratan sulfate)

产品编码 R0243
外观与形状(20°C) 液体
储存温度 冷冻 (-20°C)
应避免的情况 加热
技术规格
Appearance Colorless to Almost colorlessclear liquid
Titer min. 100 mU/Vial
物性(参考值)
GHS
相关法规
新化学物质备案回执号 B1A232224108
运输信息
监管条件代码(*)
应用
Endoglycoceramidase

References


文章/手册

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