rGRFT-Biotin [for Manα(1-2)Man]

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纯度/分析方法:

别名：

• rGRFT-生物素 [for Manα(1-2)Man]
• rGRFT-生物素结合物
• 重组凋毛藻凝集素(= rGRFT)-生物素结合物
• rGRFT-Biotin Conjugate
• Recombinant Griffithsia sp. lectin (= rGRFT)-Biotin Conjugate
• Recombinant Griffithsin-Biotin Conjugate

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规格	单价	上海	天津	日本*	数量	库存详情
1ML	¥990.00	从天津仓库发货	1	13		查询

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Product Information
Product code: R0234
Product name: rGRFT-Biotin [for Manα(1-2)Man]
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Biotin-immobilized recombinant GRFT, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: >13 kDa
Preservative : None
Stabilizer: None

Description
The rGRFT is a carbohydrate-binding protein (Lectin) derived from Griffithsia sp. The rGRFT gene product is expressed in Escherichia coli. This lectin preferably binds to a [Manα(1-2)Man] glycan epitope.
Biotin is covalently linked to the protein surface. Resulting band shift caused by biotinylation is checked by SDS-PAGE. (Unbiotinylated band is mostly not observed.) The rGRFT -Biotin shows significant interaction with bovine RNase B possessing [Manα(1-2)Man] glycan epitopes by lectin-ELISA analysis. According to the lectin-blotting analysis, the rGRFT-Biotin specifically binds to α(1-2)Man-terminated bovine RNase B but not to unmannosylated glycoproteins such as human transferrin (hTF), asialo hTF and agalacto hTF.

Storage
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).

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