Sialidase (Neuraminidase) Agarose

×

Pureté:

Synonymes

Documents de produit:

SDS | Spécifications | CoA et autres Certificats | Graphiques analytiques

Informations générales | Spécifications et Propriétés | Sécurité et Réglementaire | Applications & Litterature

Informations supplémentaires sur le produit

Taille	Prix ​​unitaire	Belgique	Japon *	Quantité
1VIAL	€422.00	Contactez-nous	5

*Le délai de livraison pour des produits disponibles en stock en Belgique est 1 à 2 jours
*Le délai de livraison pour des produits disponibles en stock en Japon est 1 à 2 semaines (sauf des produits réglementés et des envois avec de la glace carbonique)

Ajouté à votre panier d'achat

Ajouter au panier

Devis en Vrac

Product Information

• Preparation : Sialidase is covalently coupled with agarose gel
• Quantity : 0.1 mL gel/0.2 mL (1:1, v/v slurry) suspended in Storage buffer
• Activity : >10 U/mL gel
• Storage buffer : 20 mM Sodium phosphate buffer, pH 7.4
• Preservative : 0.02% [w/v] Sodium azide
• Stabilizer : None

Description

• The Sialidase Agarose is an enzyme-immobilized agarose.
  This Sialidase is a glycoside hydrolase that cleaves the glycosidic linkages of neuraminic acids. The Sialidase acts on the α2-3, α2-6, and α2-8 glycosidic linkages located on terminal sialic acid residues composed of oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrates. This enzyme is derived from Arthrobacter ureafaciens.

Unit Definition

• One unit (U) of the Sialidase is defined as the amount required to produce 1 μmol of N- acetylneuraminic acid (Neu5Ac) from Neu5Ac-α-4MU (Product No. M3031) per minute at 37°C under the condition of pH 5.0.

Assay Conditions

• Reaction conditions: 50 mM Sodium acetate, pH 5.0
  The reaction mixture includes the enzyme-immobilized agarose and Neu5Ac-α-4MU in 200 μL. The mixture is incubated at 37°C for 10 minutes. After the enzyme reaction, quenching is performed by adding a 4-fold volume of 0.1 M sodium carbonate buffer, pH 10.0. Quantification of released 4-MU is measured by HPLC.

Storage

• Store at 4°C. (Avoid freezing).

Related Products

• G0622 O-Glycosidase for core1 (Endo-α and Sialidase mix)
• I1205 Sialidase (Neuraminidase)
• 
  
• Other oligosaccharide-releasing enzymes
• [Endo-type N-glycanase (ENGase)]
• A1651 Endo-M (for hydrolysis of non core-fucosylated biantennary N-glycan)
• E1339 Endo-M-W251N (for hydrolysis of core-fucosylated biantennary N-glycan)
• G0365 Glycosynthase (for transglycosylation of non core-fucosylated biantennary N-glycan)
• 
  
• [Endo-type O-glycanase]
• A1844 Endo-α (for hydrolysis of core1 O-glycan epitope known as T/TF antigen)
• 
  
• [Endo-type glycoceramidase]
• R0240 rEGCase I
• R0241 rEGCase I assisted by Activator II
• R0242 rEGCase II
• R0243 rEGCase II assisted by Activator II
• 
  
• [Endo-type glycosaminoglycanase]
• K0069 Keratanase II (for hydrolysis of Keratan sulfate)