NBD-PZ (=4-Nitro-7-piperazino-2,1,3-benzoxadiazole) [for HPLC Labeling]

The compound 1 is an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and a piperazino group, easily reacts with a carboxyl group at room temperature to form the corresponding amide in the presence of a condensation reagent. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 470 nm and 541 nm, respectively. Since their excitation and fluorescence wavelengths are at longer wavelengths, detection has less interference by contaminants. A highly sensitive detection can be done by using laser induced fluorescence detector.

Application examples:
[Fatty acids] ¹⁾
0.2 mL of 140 mM DEPC or 70 mM 2,2’-dipyridyl disulfide-Ph₃P / DMF solution containing a fatty acid (10 µM) is added to 0.2 mL of the labeling reagent 1 / DMF or acetonitrile solution (10 mM). React at room temperature for 6 h, then use it as an HPLC sample.

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )