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Chemiluminescence Enhancer
No.155(November 2012)
In research for life-science, one of the frequently used chemiluminescence (CL) reagents is luminol. Luminol reacts with hydrogen peroxide in the presence of a catalyst, for example, enzymes, metals such as iron, or metal-complexes to emit strong light. In the case of luminol, blue light with a wavelength of 460 nm is emitted.
Since Thorpe et al. reported that phenolic compounds such as 4-iodophenol enhance CL by a peroxidase-luminol-hydrogen peroxide reaction,1) many improved methods have been reported and are called enhanced chemiluminescence (ECL). ECL is one of the sensitive and rapid detection methods in medical and analytical biochemistry.2,3)
References
- 1)Phenols as enhancers of the chemiluminescent horseradish peroxidase-luminol-hydrogen peroxide reaction: application in luminescence-monitored enzyme immunoassays
- 2)Chemiluminescent and bioluminescent methods in analytical chemistry
- 3)Enhanced chemiluminescence enzyme immunoassay
- 4)p-Iodophenol-enhanced luminol chemiluminescent assay applied to discrimination between acute lymphoblastic and minimally differentiated acute myeloid (FAB-M0) or acute megakaryoblastic (FAB-M7) leukemias
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