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Published TCIMAIL newest issue No.197
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CAS RN: | Product Number: R0227
Recombinant Marasmius oreades agglutinin (= rMOA) expressed in Escherichia coli
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Purity:
Synonyms:
- Recombinant Marasmius oreades lectin (= rMOA)
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
|---|---|---|---|---|
| 1ML |
146,00 €
|
Contact Us | 11 |
|
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
Supplemental Product Information:
[Handling Notes]
Avoid multiple freeze-thaw cycles. Add some preservative, e.g. sodium azide upon opening for the first time, and store at 2-10 °C.
Product Information
Product code: R0227
Product name: Recombinant Marasmius oreades agglutinin (= rMOA) expressed in Escherichia coli
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Purified recombinant MOA, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: 32 kDa
Preservative : None
Stabilizer: None
Description
The rMOA is a carbohydrate-binding protein (Lectin) derived from Marasmius oreades. The rMOA gene product is expressed in Escherichia coli. This lectin preferably binds to a [Galα(1-3)Gal] glycan epitope. Consequently, rMOA exhibits a hemagglutination activity to a rabbit reticulocyte through the glycan binding to subsites and subunits of the lectin.
Storage
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it's recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).
Related Products
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R0237 rMOA-LecBeads [for Galα(1-3)Gal]
R0225 Recombinant Polyporus squamosus lectin (=rPSL1a) expressed in Escherichia coli
R0226 Recombinant Laetiporus sulphureus lectin N-Terminal Domain (= rLSL-N) expressed in Escherichia coli
R0228 Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in Escherichia coli
R0229 Recombinant Griffithsia sp. lectin (= rGRFT) expressed in Escherichia coli
L0169 Lectin, Fucose specific (= AOL) from Aspergillus oryzae (5mg/mL, PBS pH6.5)
| Product Number | R0227 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Frozen (-20°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Light yellow to Light orange clear liquid |
| Concentration(Lowry method) | 0.9 to 1.5 mg/mL |
| Molecular weight | 25000 to 40000(SDS-PAGE) |
| Hemagglutination activity | max. 12.5 micro g/mL(pH7.2) |
Properties (reference)
GHS
Related Laws:
Transport Information:
| HS Number | 3504009000 |
Application
Recombinant lectin
References
- Comparison of the binding properties of the mushroom Marasmius oreades lectin and Griffonia simplicifolia I-B4 isolectin to αgalactosyl carbohydrate antigens in the surface phase
- Cloning, expression, and characterization of the Galα1,3Gal high affinity lectin from the mushroom Marasmius oreades
- Structural characterization of a lectin from the mushroom Marasmius oreades in complex with the blood group B trisaccharide and calcium
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