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CAS RN: | Product Number: N1113
Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]
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Purity:
Synonyms:
- NBT / X-Phosphate p-Toluidine Salt Solution (50X)
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
|---|---|---|---|---|
| 5ML |
€85.00
|
Contact Us | 9 |
|
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
Supplemental Product Information:
Product Information
Volume : 5 mLDescription
This product is supplied as a concentrated solution containing nitro blue tetrazolium (NBT) and 5-Bromo-4-chloro-3-indolyl phosphate (X-Phosphate p-Toluidine Salt) for Western Blotting. When this product is reacted with alkaline phosphatase (ALP), a blue-purple precipitate appears.
Direction for Use
- Incubate a blotting membrane with an ALP-conjugated antibody and wash the membrane.
- Dilute NBT / X-Phosphate p-Toluidine Salt solution (Product No. N1113) to 1X before use.
(Dilute solution : 20 mM Tris-HCl pH 9.5, 2mM MgCl2 and 100 mM NaCl) - Incubate the washed membrane with 1X NBT / X-Phosphate p-Toluidine Salt solution until color development.
- Add deionized water to stop the color development.
Storage
Store at 4 °C. Do not freeze.
Related Products
D5909 DAB staining kitB1239 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt [for Biochemical Research]
D0844 Nitro Blue Tetrazolium [for Biochemical Research]
C3384 4-Chloro-1-naphthol (Ready-to-use solution) [for Western blotting]
N1109 4-Nitrophenyl Phosphate (Ready-to-use solution) [for ELISA]
| Product Number | N1113 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
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