Published TCIMAIL newest issue No.198
Maximum quantity allowed is 999
CAS RN: | Product Number: G0615
Gel Negative Stain kit [for Electrophoresis]
Purity:
- Zinc Stain kit
Size | Unit Price | Belgium | Japan* | Quantity |
---|---|---|---|---|
1KIT |
€103.00
|
Contact Us | 22 |
|
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
Supplemental Product Information:
Product Information
Components : 10X Negative Staining Solution A, 10X Negative Staining Solution B, 10X Negative De-Staining Solution CSize : 20 tests
Description
Imidazole-Zinc Reverse Staining is a detection method for SDS-PAGE separated proteins, in which only regions of gel not containing proteins are stained white, while protein-containing regions remain transparent. Bands are visualized by placing the gel against a dark background. Destaining can be achieved using the Negative De-Staining Solution C, allowing proteins to be eluted or transferred to a membrane.
Storage
Store at room temperature and used within 12 months.
Direction for Use
Preparation (For 90 x 90 x 1mm gel)
The amount of reagent to adjust depends on the gel size.
Solution B : Prepare Solution B by mixing 5 mL of 10X Negative Staining Solution B with 45 mL of de-ionized water. (Note: This solution contains zinc sulfate. Check your local laws for disposal of Zinc.)
Solution C : Prepare Solution C by mixing 5 mL of 10X Negative De-Staining Solution C with 45 mL of de-ionized water.
Preservative solution : Prepare by mixing 200 μL of 10X Negative Staining Solution A with 50 mL of de-ionized water.
- Place gel in a properly-sized container with 50mL of deionized water and shake for 5 - 10 minutes.
- Remove deionized water, add 50mL Solution A, and shake 5min.
- Remove Solution A and rinse the gel 3 times (10 seconds) with 50mL of deionized water. (Note: Rinsing for too long will lead to poor staining results.)
- Place gel in another container, add 50mL Solution B, and shake for 30 - 100 seconds. Note: This step must not be extended longer than 100s or band overstaining and loss of the image will occur.
- Transfer the gel to the container used in step 3, add 50mL deionized water, and rinse as soon as the bands appear. Rinse the gel 3 times with water for 30 – 60 seconds. Photograph immediately afterwards.
- Photograph the gel. It is recommended that the gel be photographed on a black tray. Note: Gels can be stored in Preservative solution, but should be photographed as soon as possible. Stained gels cannot be dried and stored.
- Remove deionized water, add 50mL Solution C, and shake until stain is removed.
- Remove Solution C and rinse the gel 3 times (30 seconds) with 50mL of deionized water.
- After destaining, proteins are free of dyes and other contaminants. As such, they can be transferred to a membrane for Western blotting or eluted from excised gel pieces.
Product Number | G0615 |
Storage Temperature | Room Temperature (Recommended in a cool and dark place, <15°C) |
Articles/Brochures
Safety Data Sheet (SDS)
The requested SDS is not available.
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Specifications
C of A & Other Certificates
Sample C of A
A sample C of A for this product is not available at this time.
Analytical Charts
The requested analytical chart is not available. Sorry for the inconvenience.