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CAS RN: | Product Number: B5834
2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
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Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
|---|---|---|---|---|
| 25ML |
€56.00
|
1 | ≥80 |
|
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
Supplemental Product Information:
Product Information
Volume : 25 mLDescription
Preparation of non-reducing sample
A protein sample is mixed with the 2X SDS-PAGE sample buffer (1:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.
Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 10 μL of 2-Mercaptoethanol per 90 μL of 2X SDS-PAGE sample buffer. Mix thoroughly. Then a protein sample is mixed with the sample buffer (1:1) and heating to 95–100°C for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (Product No. M1948), DL-Dithiothreitol [for Electrophoresis] (Product No. D3647), etc.
Storage
Store at room temperature.
Related Products
C3488 Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]G0615 Gel Negative Stain kit [for Electrophoresis]
D3647 DL-Dithiothreitol [for Electrophoresis]
M1948 2-Mercaptoethanol [for Electrophoresis]
A3217 30% Acrylamide / Bis-acrylamide (29:1) [for Electrophoresis]
A3218 30% Acrylamide / Bis-acrylamide (37.5:1) [for Electrophoresis]
B6104 4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6105 6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6110 2X SDS-PAGE Sample Buffer Phenol Red (2-Mercaptoethanol free) [for Electrophoresis]
| Product Number | B5834 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Room Temperature (Recommended in a cool and dark place, <15°C) |
Specifications
| Appearance | Green to Dark green to Dark blue clear liquid |
| Functionality test | Electrophoresis to pass test |
Properties (reference)
GHS
Related Laws:
Transport Information:
| HS Number | 3822190090 |
Application
2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
Reference
- Cleavage of structural proteins during the assembly of the head of bacteriophage T4
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