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CAS RN: | Product Number: A2659
AOL-Biotin Conjugate
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Synonyms:
- Aspergillus oryzae lectin (= AOL)-Biotin Conjugate
- AOL-biotin [for Fucα(1-6)/α(1-4)/α(1-3)/α(1-2)]
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
|---|---|---|---|---|
| 1ML |
337,00 €
|
1 | 19 |
|
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
Supplemental Product Information:
Product Information
Quantity : 1 ml per vial (1 mg/1 ml: Lowry method)Product Form : Biotin-immobilized recombinant AOL, Liquid
Buffer : 50 mM Phosphate buffer, pH6.5
Molecular Mass : >35 kDa
Preservative : None
Stabilizer : None
Description
The AOL is a carbohydrate-binding protein (Lectin) derived from Aspergillus oryzae. The AOL gene product is expressed in Aspergillus oryzae. (The gene is derived from the same origin as the host cell.) This lectin preferably binds to a [Fucα(1-6)/α(1-4)/α(1-3)/α(1-2)] glycan epitopes.
Biotin is covalently linked to the protein surface. Resulting band shift caused by biotinylation is checked by SDS-PAGE. (Unbiotinylated band is mostly not observed.) The AOL-Biotin Conjugate shows significant interaction with human lactoferrin (hLF) possessing [Fucα(1-6)/α(1-3)] glycan epitopes by lectin-ELISA analysis. According to the lectin-blotting analysis, the AOL-Biotin Conjugate specifically binds to α(1-6/1-3)Fucsylated hLF but not to unfucosylated glycoproteins such as human transferrin (hTF), asialo hTF, agalacto hTF and bovine RNase B.
Storage
Store at 2-10°C.
Related Products
L0169 Lectin, Fucose specific (= AOL) from Aspergillus oryzae (5 mg/ml, PBS pH6.5)A3331 AOL-LecBeads [for Fucα(1-6)/α(1-4)/α(1-3)/α(1-2)]
R0235 rPSL1a-LecBeads [for Siaα(2-6)Gal]
R0236 rLSL-N-LecBeads [for Galβ(1-4)GlcNAc, poly LacNAc]
R0237 rMOA-LecBeads [for Galα(1-3)Gal]
R0238 rSRL-LecBeads [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0239 rGRFT-LecBeads [for Manα(1-2)Man]
| Product Number | A2659 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Light yellow clear liquid |
| Concentration(Lowry method) | 0.9 to 1.5 mg/mL |
| Molecular weight | 30 to 40 kDa |
| Suitability for use in ELISA tests | min. 1.0 (human Lactoferrin, 10 micro g/mL, OD450) |
| Fucose inhibition test | to pass test |
Properties (reference)
GHS
Related Laws:
Transport Information:
| HS Number | 3002909010 |
Application
Fucose-specific lectin
References
- Molecular cloning and overexpression of fleA gene encoding a fucose-specific lectin of Aspergillus oryzae
- Carbohydrate binding specificity of a fucose-specific lectin from Aspergillus oryzae: a novel probe for core fucose
- Six independent fucose-binding sites in the crystal structure of Aspergillus oryzae lectin
Application
Biotinylated Fucose-specific Lectin
References
- Carbohydrate binding specificity of a fucose-specific lectin from Aspergillus oryzae
Articles/Brochures
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