Published TCIMAIL newest issue No.196
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Detergent for Biochemical Research
No.165(April 2015)
Tris dodecyl sulfate (1) is one of the anionic detergents which contains dodecyl sulfate. The property of 1 is different from that of sodium dodecyl sulfate (SDS). 1 has several advantages for biochemical analysis over SDS as described below.
Booth reported that, on two-dimensional electrophoresis of membrane proteins, 1 gives good resolution.1) Kubo and Takagi reported the effect of alkanolammonium dodecyl sulfates for Weber and Osborn’s polyacrylamide gel electrophoresis system.2) The abilities of the dodecyl sulfates as to the dissociation of the molecular assemblies of chlorophyll-proteins were found to decrease in the following order: 1 > triethanolammonium salt > triisopropanolammonium salt.
Depending upon antigens and antibodies, 1 gives different reactivities from SDS on immunoblotting.3) 1 was also utilized for chromatographical separation of rat tail tendon collagen from monomeric components.4)
References
- 1)A novel system for the two-dimensional electrophoresis of membrane proteins
- 2)Marked effect of cations on the separation of spinach thylakoid chlorophyll-proteins on polyacrylamide gel electrophoresis at 0 °C in the presence of alkanolammonium dodecyl sulfates
- 3)Chlamidia pecorum – The fourth species of genus Chlamydia –
- 4)Separation of cross-linked products of rat tail tendon collagen from monomeric components by gel chromatography in the presence of tris dodecyl sulfate at 4 °C
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