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CAS RN: | Product Number: T3940
Tissue-Clearing Reagent iTOMEI-D [for Plants]
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Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
|---|---|---|---|---|---|
| 5ML |
$32.00
|
Contact Us | Contact Us | 13 |
|
| 25ML |
$95.00
|
2 | 1 | 1 |
|
* Items in stock locally ship in 1-2 business days. Items from Japan stock are able to ship from a US warehouse within 2 weeks. Please contact TCI for lead times on items not in stock. Excludes regulated items and items that ship on ice.
* To send your quote request for bulk quantities, please click on the "Request Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Supplemental Product Information:
"Improved TOMEI (iTOMEI)" is a plant tissue-clearing method that specializes TOMEI for fluorescent protein observation by Prof. Sakamoto et al. iTOMEI enables simply transparency and clearly detecting fluorescent protein.
This product is a decoloring reagent for iTOMEI.
Please see a brochure (PDF) of how to use.
Description
This product enables transparency by simple osmosis in just a few days (more than 2 days) and keeps the fluorescence of a fluorescent protein such as GFP or tdTomato co-expressed as a reporter gene.
The mounting solution is also required to complete the iTOMEI method.
Please check directions for use for necessary reagents.
Storage
The product should be stored at 4 °C.
Directions for Use
Required reagents;
- Paraformaldehyde (PFA)
- PBS
- Stains* (option)
- Mounting solution**
==Process outline (All operations are performed at Room temperature)===============
- Tissue fixation
Reagent : 1% PFA in PBS
Time : 1hr - PBS Wash ×3
- Decoloring
Reagent : iTOMEI-D (TCI Product No. T3940)
Time : 24hr - PBS Wash ×3
- (Optional process Staining*, PBS Wash ×3)
- Clearing (RI matching)
Reagent : Mounting solution**
Time : Operation time is determined by the mounting solution. - Mounting
Reagent : Mounting solution**
* The treatment time of DAPI staining is for 30min with 5 µg/mL and the treatment time of Calcofluor White staining is for 10min with 1 g/L of Calcofluor White M3R and 0.5 g/L of Evans Blue, but their adjustments are needed for the purpose.
** Mounting solution : Please use the suitable mounting solution for objective lens.
Related Products
P0018 Paraformaldehyde
A2412 DAPI·2HCl
E0197 Evans Blue
A0132 Acridine Orange
A0321 Tris-Base
G0316 Glycerol [for Electrophoresis]
S0111 D-(+)-Sucrose
T3530 Tissue-Clearing Reagent TOMEI [for Plants]
| Product Number | T3940 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Almost colorless clear liquid |
| Functionality test(fluorescence retention) | to pass test |
| Functionality test(Tissue bleaching) | to pass test |
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H315 : Causes skin irritation. H319 : Causes serious eye irritation. |
| Precautionary Statements | P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P302 + P352 : IF ON SKIN: Wash with plenty of soap and water. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. P362 : Take off contaminated clothing and wash before reuse. |
Related Laws:
Transport Information:
| HS Number | 3822.19.0080 |
Application
The Decoloring Solution for Plant Tissue-Clearing Method iTOMEI
Figure. Cleared Arabidopsis thaliana by “iTOMEI”
(left) Non-treatment (right) iTOMEI treatment
References
- 1) Improved clearing method contributes to deep imaging of plant organs
- 2) Whole-Tissue Three-Dimensional Imaging of Rice at Single-Cell Resolution
