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CAS RN: | Product Number: T3854

TMB [for ELISA] (Ready-to-use solution)

× TMB [for ELISA] (Ready-to-use solution)

Purity:
Synonyms:
  • 3,3',5,5'-Tetramethylbenzidine [Soluble chromogenic substrate for ELISA] (Ready-to-use solution)
Product Documents:
SDS | Specifications | C of A & Other Certificates | Analytical Charts

General Information | Specifications & Properties | Safety & Regulations | Applications

Supplemental Product Information
100ML
$75.00
 1   1   30  

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Supplemental Product Information:

Product Information
Volume : 100 mL

Description
This product is supplied as a ready-to-use solution containing 3,3',5,5'-Tetramethyl -benzidine (TMB) and hydrogen peroxide for ELISA. When this product reacts with horseradish peroxidase (HRP), a blue colored soluble reaction product appears. Its absorbance can be measured at 650 nm. After terminating the reaction by adding hydrochloric acid, the reaction product becomes yellow and its absorbance can be measured at 450 nm.

Direction for Use
The following steps are for a 96-well plate format.
1. Bring TMB solution (Product No. T3854) to room temperature.
2. Gently mix the TMB solution (Product No. T3854).
3. Add 100uL of TMB solution (Product No. T3854) to each well.
4. Incubate the plate at room temperature for 30 minutes.
5. To terminate the reaction, add 100uL of 1 N HCl (Product No. H1202) to each well.
6. Within 1 hour of terminating the reaction, measure the absorbance of each well at 450 nm.

Storage
The product should be stored at 4 ºC, and used within 6 months.
Precipitations sometimes arise, but they do not affect the quality.

Related Products
T3855 TMB (Ready-to-use solution) [for Western blotting]
T2573 3,3',5,5'-Tetramethylbenzidine [for Biochemical Research]
H1202 Hydrochloric Acid (1mol/L)
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A3187 Anti-Protein A Chicken Polyclonal Antibody HRP Conjugate
A3195 Anti-α Gal Chicken Polyclonal Antibody HRP Conjugate
G0407 Goat Anti-Mouse IgG HRP Conjugate
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G0418 Goat Anti-Rabbit IgG HRP Conjugate
P2466 Protein A HRP Conjugate
S0972 Streptavidin HRP Conjugate
S0999 Sheep Anti-Chicken IgY HRP Conjugate

Product Number T3854
Physical State (20 deg.C) Liquid
Storage Temperature Refrigerated (0-10°C)
Condition to Avoid Heat Sensitive
Specifications
Appearance Colorless to Almost colorlessclear liquid
Peroxidase detection test to pass test
Properties (reference)
GHS
Pictogram Pictogram
Signal Word Danger
Hazard Statements H370 : Causes damage to organs.
H372 : Causes damage to organs through prolonged or repeated exposure.
H227 : Combustible liquid.
Precautionary Statements P501 : Dispose of contents/ container to an approved waste disposal plant.
P270 : Do not eat, drink or smoke when using this product.
P260 : Do not breathe mist or vapours.
P210 : Keep away from heat/sparks/open flames/hot surfaces. No smoking.
P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
P307 + P311 : IF exposed: Call a POISON CENTER or doctor/ physician.
P403 + P235 : Store in a well-ventilated place. Keep cool.
P405 : Store locked up.
Related Laws:
Transport Information:
HS Number 3822.19.0080
Application
TMB [for ELISA] (Ready-to-use solution)

References

  • Cold Spring Harbor Laboratory Press, New York
    • E. Harlow and D. Lane., Antibodies, A Laboratory Manual, 1988, p404, p506, p594


Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
C of A & Other Certificates
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