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CAS RN: 9001-67-6 | Product Number: I1205
Sialidase from Arthrobacter ureafaciens
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Purity:
Synonyms:
- Neuraminidase from Arthrobacter ureafaciens
- α2-3,6,8 Sialidase from Arthrobacter ureafaciens
Product Documents:
| Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
|---|---|---|---|---|---|
| 1VIAL |
$177.00
|
Contact Us | Contact Us | 7 |
|
* Items in stock locally ship in 1-2 business days. Items from Japan stock are able to ship from a US warehouse within 2 weeks. Please contact TCI for lead times on items not in stock. Excludes regulated items and items that ship on ice.
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Supplemental Product Information:
Product Information
- Quantity : 1.0 U per vial (10 U/mL)
- Product Form : Purified Sialidase, Liquid
- Storage buffer : 20 mM Sodium phosphate buffer, pH 7.4
- Preservative : None
- Stabilizer : None
Description
- This Sialidase is a glycoside hydrolase that cleaves the glycosidic linkages of neuraminic acids. The Sialidase acts on the α2-3, α2-6, and α2-8 glycosidic linkages located on terminal sialic acid residues composed of oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrates. This enzyme is derived from Arthrobacter ureafaciens.
Unit Definition
- One unit (U) of the Sialidase is defined as the amount required to produce 1 μmol of N-acetylneuraminic acid (Neu5Ac) from Neu5Ac-α-4MU (Product No. M3031) per minute at 37°C under the condition of pH 5.0.
Assay Conditions
- Reaction conditions: 50 mM Sodium acetate, pH 5.0
The reaction mixture includes the enzyme and Neu5Ac-α-4MU in 40 μL. The mixture is incubated at 37°C for 10 minutes. After the enzyme reaction, quenching is performed by adding a 4-fold volume of 0.1 M sodium carbonate buffer, pH 10.0. Quantification of released 4-MU is measured by HPLC.
Storage
- Store at -20°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 4°C and use it within a few weeks.
(For long-term use, aliquot and freeze after thawing.)
Related Products
- G0622 O-Glycosidase for core1 (Endo-α and Sialidase mix)
- I1206 Sialidase (Neuraminidase) Agarose
- Other oligosaccharide-releasing enzymes
- [Endo-type N-glycanase (ENGase)]
- A1651 Endo-M (for hydrolysis of non core-fucosylated biantennary N-glycan)
- E1339 Endo-M-W251N (for hydrolysis of core-fucosylated biantennary N-glycan)
- G0365 Glycosynthase (for transglycosylation of non core-fucosylated biantennary N-glycan)
- [Endo-type O-glycanase]
- A1844 Endo-α (for hydrolysis of core1 O-glycan epitope known as T/TF antigen)
- [Endo-type glycoceramidase]
- R0240 rEGCase I
- R0241 rEGCase I assisted by Activator II
- R0242 rEGCase II
- R0243 rEGCase II assisted by Activator II
- [Endo-type glycosaminoglycanase]
- K0069 Keratanase II (for hydrolysis of Keratan sulfate)
| Product Number | I1205 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Frozen (-20°C) |
| Condition to Avoid | Heat Sensitive |
| CAS RN | 9001-67-6 |
Specifications
| Appearance | Colorless to Almost colorlessclear liquid |
| Titer | min. 1.0 unit/vial |
| Molecular weight | 88, 66, 52 kDa |
Properties (reference)
GHS
Related Laws:
Transport Information:
| HS Number | 3507.90.7000 |
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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