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CAS RN: | Product Number: H1621

Horseradish Peroxidase Maleimide Conjugate (0.5mg×3)

Purity:

Synonyms:

HRP Maleimide (0.5mg×3)
Horseradish Peroxidase Maleimide Activated (0.5mg×3)

Product Documents:

Size	Unit Price	Philadelphia, PA	Portland, OR	Japan*	Quantity	Shipping Information
1SET	$175.00	Ships from Portland the same day*	1	17		Shipping Simulation

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* Items in stock locally typically ship the same day of ordering. Items from Japan stock are able to ship from a US warehouse within 2 weeks after ordering. For additional estimated shipment times, please refer to the Shipping Simulation tool. Note: Excludes regulated items and items that ship on ice.
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*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.

Supplemental Product Information:

Product Information

Volume/Quantity : 0.5 mg x 3 vial
Conjugation : Peroxidase from Horseradish (HRP) was conjugated to Sulfo-SMCC (Product No. S0883).
Product Form : Horseradish Peroxidase Maleimide Conjugate. Lyophilized in 10 mM sodium phosphate, 0.115 mM NaCl, 40 mM sucrose, pH 6.6.
Preservative : None

Description

HRP Maleimide Conjugate is designed to facilitate the conjugation of the HRP to proteins, peptides and ligands that contain a free sulfhydryl (-SH) groups. Activated HRP contains 1–3 maleimide groups for each HRP molecule.

Protocol

Firstly return the vial to room temperature.
Reconstitute the contents of the vial with 100μL of buffer or water to obtain a 5 mg/mL solution of HRP Maleimide.
Note: Do not vortex or heat the HRP Maleimide.
Dissolve the sulfhydryl compound to be conjugated at 2-5 mg/ml in the buffer.
Note: We recommend that the thiol-containing molecule is dissolved in the pH range 6.5-7.5 buffer. 0.1M sodium phosphate, 0.15M NaCl, 10mM EDTA at pH 7.2 is commonly employed. Maleimides react with sulfhydryls at pH 6.5-7.5 to form stable thioether bonds. EDTA may help to prevent metal catalysed oxidation of thiols. Use of a 4:1 (HRP Maleimide:antibody) molar ratio in the conjugation reaction usually results in high-activity conjugates. This vial contains 0.5 mg of HRP Maleimide Conjugate and can effectively modify 0.5 mg of immunoglobulin.
Immediately mix the thiol-containing molecule and HRP Maleimide solutions and allow them to react for 2 hours at room temperature. To increase HRP incorporation, extend reaction time up to 12 hours.
If desired, desalt, dialyze or purify the conjugate.

Storage

Store at −20 °C to −80 °C. Reconstitute this reagent immediately before use. Do not prepare stock solutions for storage. Discard any unused reconstituted reagent.