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CAS RN: 127274-91-3 | Product Number: D6263
DiD Solution [for Cell Membrane Staining]
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![DiD Solution [for Cell Membrane Staining]](/_ui/responsive/theme-tci/images/missing_product_zoom.jpg "DiD Solution [for Cell Membrane Staining]")
Purity:
Synonyms:
- 2-[5-(3,3-Dimethyl-1-octadecylindolin-2-ylidene)penta-1,3-dien-1-yl]-3,3-dimethyl-1-octadecyl-3H-indol-1-ium Perchlorate Solution
Product Documents:
| Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
|---|---|---|---|---|---|
| 1ML |
$252.00
|
Contact Us | Contact Us | 14 |
|
* Items in stock locally ship in 1-2 business days. Items from Japan stock are able to ship from a US warehouse within 2 weeks. Please contact TCI for lead times on items not in stock. Excludes regulated items and items that ship on ice.
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*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Supplemental Product Information:
Product Information
- Concentration : 1 mM
- Excitation / Emission : 657 nm / 678 nm
Description
- DiD is a lipophilic carbocyanine dye used to label cell membranes. It emits strong fluorescence when its long hydrocarbon chains are incorporated into a lipid bilayer. This product is a ready-to-use solution of DiD which can be used as a tracer in cell fusion, cell adhesion and chemotaxis.
Storage
- Store at room temperature and protect from light.
Directions for Use: Staining live cells
-
- Dilute DiD solution to 5 μM with culture medium.
- Remove medium from cells and replace with 5 μM DiD-containing medium.
- Incubate cells at 37 °C for 10 minutes.
- Remove medium and wash cells 3 times with PBS.
- Observe stained cell membranes via a fluorescence microscope.
Directions for Use: Staining fixed cells
-
- Remove culture medium and wash cells twice with PBS.
- Add 4% paraformaldehyde to cells and incubate at room temperature for 10 minutes.
- Dilute DiD solution to 5 μM with PBS.
- Remove 4% paraformaldehyde and add 5 μM DiD-containing PBS to cells.
- Incubate cells at 37 °C for 10 minutes.
- Remove medium and wash cells 3 times with PBS.
- Observe stained cell membranes via a fluorescence microscope.
| Product Number | D6263 |
| Molecular Formula / Molecular Weight | C__6__1H__9__9ClN__2O__4 = 959.92 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Room Temperature (Recommended in a cool and dark place, <15°C) |
| CAS RN | 127274-91-3 |
Specifications
| Appearance | Blue to Dark blue clear liquid |
| Cell staining test | to pass test |
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Danger |
| Hazard Statements | H319 : Causes serious eye irritation. H360 : May damage fertility or the unborn child. H372 : Causes damage to organs through prolonged or repeated exposure. H373 : May cause damage to organs through prolonged or repeated exposure. H340 : May cause genetic defects. H350 : May cause cancer. H402 : Harmful to aquatic life. H225 : Highly flammable liquid and vapor. |
| Precautionary Statements | P501 : Dispose of contents/ container to an approved waste disposal plant. P273 : Avoid release to the environment. P270 : Do not eat, drink or smoke when using this product. P202 : Do not handle until all safety precautions have been read and understood. P240 : Ground/bond container and receiving equipment. P260 : Do not breathe mist or vapours. P210 : Keep away from heat/sparks/open flames/hot surfaces. No smoking. P233 : Keep container tightly closed. P201 : Obtain special instructions before use. P243 : Take precautionary measures against static discharge. P241 : Use explosion-proof electrical/ ventilating/ lighting/ equipment. P242 : Use only non-sparking tools. P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection. P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish. P308 + P313 : IF exposed or concerned: Get medical advice/ attention. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P303 + P361 + P353 : IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower. P403 + P235 : Store in a well-ventilated place. Keep cool. P405 : Store locked up. |
Related Laws:
Transport Information:
| UN Number (DOT-AIR) | UN1993 |
| Class (DOT-AIR) | 3 |
| Packing Group (TCI-A) | II |
| HS Number | 3822.19.0080 |
Articles/Brochures
TCIMAIL
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
C of A & Other Certificates
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