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CAS RN: | Product Number: D5909
DAB staining Kit
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Product Documents:
| Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
|---|---|---|---|---|---|
| 1KIT |
$249.00
|
Contact Us | Contact Us | 5 |
|
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Supplemental Product Information:
Product Information
Components : DAB solution, Hydrogen Peroxide solution, 1.5M Tris-HCl buffer
Size : 100 tests (About 500 mL substrate solution)
Description
This kit consists three reagents; 3, 3’-diaminobenzidine (DAB) solution, Hydrogen Peroxide solution and 1.5M Tris-HCl buffer and it is supplied to detect horseradish peroxidase (HRP) activity in Western blotting and so on. When DAB reacts with HRP, a brown precipitate appears. This kit does not include nickel component.
Direction for Use
1) Prepare a blotting membrane covered with HRP-conjugated antibody.
2) Prepare the DAB substrate solution according to the following volume;
- Add 5 drops of DAB solution (about 140 µL)
- Add 2 drops of Hydrogen Peroxide solution (about 80 µL)
- Add 2 drops of 1.5M Tris-HCl buffer (about 80 µL)
- Each reagent is added into 5 mL of deionized water
3) Soak the membrane in the DAB substrate solution and incubate until the membrane is colored.
4) Add deionized water and stop the coloring reaction.
Storage
The product should be stored at 4 °C and used within 6 months.
| Product Number | D5909 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
Properties (reference)
GHS
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Transport Information:
| HS Number | 3822.19.0080 |
Application
DAB Staining Kit to Detect Peroxidase
Figure. Dot blot image of substrate solutions prepared by DAB staining kit reacting with HRP (from left to right: 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156, 0.078 (mU/spot)).
References
- Assessment of a method for immunochemical detection of antigen on nitrocellulose membranes.
- Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.
Application
Staining using DAB as a chromogenic substrate for peroxidase
References
- Studies on Microperoxisomes V. Are Microperoxisomes Ubiquitous in Mammalian Cells?
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Specifications
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