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CAS RN: | Product Number: B6104
4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
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Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
|---|---|---|---|---|---|
| 20ML |
$62.00
|
Contact Us | Contact Us | ≥100 |
|
* Items in stock locally ship in 1-2 business days. Items from Japan stock are able to ship from a US warehouse within 2 weeks. Please contact TCI for lead times on items not in stock. Excludes regulated items and items that ship on ice.
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Supplemental Product Information:
Product Information
- Volume : 20 mL
Description
- This product contains 8% SDS, 40% glycerol, 0.02% bromophenol blue and 0.25 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol.
SDS-PAGE Sample Buffer is specially formulated for protein sample preparation based on the Laemmli Method of SDS-PAGE system.
Note: Dissolve the precipitation at 25-30 °C if precipitation occurs during storage.
Direction for Use
- Preparation of non-reducing sample
A protein sample is mixed with the 4X SDS-PAGE sample buffer (3:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE. - Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 20 μL of 2-Mercaptoethanol per 80 μL of 4X SDS-PAGE sample buffer. Mix thoroughly. Then a protein sample is mixed with the sample buffer (3:1) and heating to 95–100°C for 5 minutes. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (Product No. M1948), DL-Dithiothreitol [for Electrophoresis] (Product No. D3647), etc.
Storage
- Store at room temperature.
Related Products
- A3217 30% Acrylamide / Bis-acrylamide (29:1) [for Electrophoresis]
- A3218 30% Acrylamide / Bis-acrylamide (37.5:1) [for Electrophoresis]
- B5834 2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
- B6105 6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
- B6110 2X SDS-PAGE Sample Buffer Phenol Red (2-Mercaptoethanol free) [for Electrophoresis]
- C3488 Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
- D3647 DL-Dithiothreitol [for Electrophoresis]
- G0615 Gel Negative Stain kit [for Electrophoresis]
- I1309 Silver Stain Kit [for Electrophoresis]
- M1948 2-Mercaptoethanol [for Electrophoresis]
| Product Number | B6104 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Room Temperature (Recommended in a cool and dark place, <15°C) |
Specifications
| Appearance | Purple to Dark purple clear liquid to cloudy liquid |
| Functionality test | Electrophoresis to pass test |
Properties (reference)
| Specific Gravity (20/20) | 1.14 |
GHS
Related Laws:
Transport Information:
| HS Number | 3822.19.0080 |
Application
4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
References
- Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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