Phenacyl Bromide [for HPLC Labeling]

The compound 1 is an HPLC labeling reagent, which has a bromoacetyl group and easily reacts with a carboxyl group to form the corresponding ester in the presence of a base. The resultant ester is stable and can reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by measuring at 254 nm, an absorption wavelength that is generally used for UV detection.

Application examples:
[Fatty acids] ¹⁾
Mix ca. 100 µg of a sample, 10 µL of the labeling reagent 1 in acetone (12 mg / mL) and 10 µL of triethylamine in acetone (10 mg / mL), and incubate the solution at 50 °C for 2 h. Cool the resultant solution to room temperature and use it as an HPLC sample.
[Others]
Bile acids²⁾, fatty acids³⁾, carboxylic acids in wine⁴⁾

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )