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Fluorescent Probe: 5-CFDA
No.173(April 2017)
Carboxyfluorescein diacetate (CFDA) is used to measure intracellular pH.1) CFDA easily permeates cells and is converted to carboxyfluorescein. The carboxyfluorescein is retained better in cells compared with fluorescein. Therefore, CFDA is applied in the cell viability assay.2)
5-Carboxyfluorescein diacetate (5-CFDA, 1) is used for the FRAP assay by combination with another fluorescent probe. In the study on the heterocyst forming of cyanobacterium by Plominsky et al., 1 and calcein were used for the FRAP assay.3)
FRAP: fluorescence recovery after photobleaching
References
- 1)Intracellular pH measurements in Ehrlich ascites tumor cells utilizing spectroscopic probes generated in situ
- 2)Carboxyfluorescein fluorochromasia cell-mediated lympholysis. A comparative study
- 3)Intercellular transfer along the trichomes of the invasive terminal heterocyst forming cyanobacterium Cylindrospermopsis raciborskii CS-505
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