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Published TCIMAIL newest issue No.197
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Rapid Transparency Reagent for Plants: TOMEI
The transparency technique "TOMEI" for plant analysis was developed by Prof. Matsunaga et al. at Tokyo University of Science. This method enables the clearing of Oryza sativa, Arabidopsis thaliana and many others in just a few hours. Using this technique, deeper internal structure can be clearly observed. TCI offers TOMEI reagent for this transparency techique.
Product
*This item is unavailable except in Japan, U.S. and Europe.Advantages
- Simple and rapid clearing of plant can be performed.
- Deeper part can be observed by using confocal microscope.
Applications
The transparency technique "TOMEI-I" reduces the intrinsic fluorescence of chlorophyll, as well as reduced fluorescent protein, and thus observation benefits from the use of fluorescent dyes.
Alternatively, transparency technique "TOMEI-II" doesn't significantly reduce intrinsic fluorescence due to its mild conditions, but the transparency is adequate to detect fluorescent proteins.
Figure 1. Cleared Oryza sativa (left) and Arabidopsis thaliana (right) by "TOMEI-I"
Figure 2. Cleared Arabidopsis thaliana by "TOMEI-II"
Figure 3. Optical sections images of root of Arabidopsis thaliana obtained using confocal microscope.
Nuclear is detected by DAPI (blue) and H2B-GFP (green), and cell membrane is detected by LTI6b–tdTomato (red).
All of a~h are images of central part of root.
Fluorescence of H2B-GFP (a, e) is detected strongly either in sample only fixation (a) and in TOMEI-II-treated sample (e).
It's difficult to detect fluorescent of DAPI (b) and LTI6b-tdTomato (c) in central part of root only fixation.
In contrast, fluorescent of DAPI (f) and LTI6b-tdTomato (g) is detected even in cells of central part of TOMEI-II-treated root.
References
- Three-Dimensional Imaging of Plant Organs Using a Simple and Rapid Transparency Technique
- The coordination of ploidy and cell size differs between cell layers in leaves
