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Published TCIMAIL newest issue No.200
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Videos explaining the Introduction and Use of Life Science Products
This page contains videos explaining experimental techniques for biochemistry using our products. The videos are especially recommended for the following people;
- Individuals interested in learning the basic experimental techniques of biochemistry.
- Beginners conducting biochemistry experiments.
- Individuals who are considering to use life science products.
Video of Protein Extraction Protocol for Mammalian Cells Using Extraction/Fractionation Reagents
The first step in protein purification, lysing tissue or cells to extract protein, is crucial for properties and yield of the obtained protein. This 4 minute video explains how to extract and measure the concentration of intracellular proteins from mammalian cells using two protocols, one for total protein, and one with an extra step which allows for fractionation of cytoplasmic/nuclear proteins.
Video of SDS-PAGE Sample Preparation Protocol Using Reducing Reagent
SDS polyacrylamide gel electrophoresis (SDS-PAGE) is often used to check purity and determine molecular weight during the protein purification process. This 3 minute video summarizes the process of preparing a sample for SDS-PAGE by treating the sample with a reducing agent and sample buffer. In addition to the reagents shown in the video, we offer a wide range of other reagents related to protein electrophoresis.
Video of Tissue clearing protocol for CUBIC reagents
A tissue clearing method "CUBIC" has been developed by Prof. Hiroki R. Ueda and coworkers at The University of Tokyo / RIKEN. The CUBIC technique enables cyclopedic imaging at a single-cell resolution following whole-body and whole-organ clearing. This 3 minute video explains how to make a mouse brain transparent by using animal tissue-clearing reagents "CUBIC".
Video of Plants tissue clearing protocol for iTOMEI reagents
The transparency technique "TOMEI" for plant analysis was developed by Prof. Matsunaga et al. This method enables the clearing of Oryza sativa, Arabidopsis thaliana and many others in just a few hours. Using this technique, deeper internal structure can be clearly observed. Improved TOMEI (iTOMEI) is a method that specializes TOMEI for fluorescent protein observation by Prof. Sakamoto et al. This method enables simple transparency and allows for clear detection of fluorescent proteins. This 3 minute video explains how to make Arabidopsis thaliana transparent by using plants tissue-clearing reagents "iTOMEI". It enables transparency by simple osmosis in just a few days.