Published TCIMAIL newest issue No.196
Maximum quantity allowed is 999
Please select the quantity
CAS RN: | Product Number: Y0021
E.coli / Yeast Protein Extraction Buffer
×
Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
|---|---|---|---|---|
| 100ML |
€90.00
|
1 | 6 |
|
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
Supplemental Product Information:
Product Information
Volume : 100 mLAdditives : WITHOUT protease inhibitors
Description
This product is supplied as a ready-to-use solution to lyse cultured E.coli / yeast cells. Extracted protein can be used in downstream applications such as Western blotting and protein quantification. This product does not include protease inhibitors.
Directions for Use: Lysis of E.coli cells
1. Centrifuge E.coli cells at 5000 × g for 10 minutes.
2. Remove as much medium as possible from pellet.
3. Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 2-4 mL buffer per gram pellet.
4. Incubate for 10 minutes at room temperature.
5. Centrifuge lysate at 15,000 × g for 10 minutes.
6. Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.
Directions for Use: Lysis of yeast cells
1. Centrifuge yeast cells at 3000 × g for 10 minutes.
2. Remove as much medium as possible from pellet.
3. Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 2-4 mL buffer per gram pellet.
4. Agitate at room temperature for 10 minutes.
5. Centrifuge lysate at 14,000 × g for 10 minutes.
6. Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.
Storage
Store at 4 °C and use within 6 months.
Related Products
A2215 AEBSF [for Biochemical Research]D3789 Disodium Dihydrogen Ethylenediaminetetraacetate Dihydrate [for Biochemical Research]
E1337 E-64d [for Biochemical Research]
P2575 Pyrogallol Red (Ready-to-use solution) [for Protein determination]
P2949 100×Protease Inhibitor Cocktail (EDTA free)
P2976 100×Protease Inhibitor Cocktail
| Product Number | Y0021 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Almost colorlessclear liquid |
| Suitability for protein-analysis (E.coli) | to pass test |
| Suitability for protein-analysis (Yeast) | to pass test |
Properties (reference)
| Specific Gravity (20/20) | 1.06 |
GHS
Related Laws:
Transport Information:
| HS Number | 3822190090 |
Application
E.coli / Yeast Protein Extraction
References
- Non-detergent sulphobetaines: a new class of mild solubilization agents for protein purification
Articles/Brochures
TCIMAIL
[Featured Products] Ready-to-use Solutions for Protein Extraction[TCIMAIL No.188] Ready-to-Use Solution for Protein Extraction from E. coli / Yeast Cells
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
Please select Language.
The requested SDS is not available.
Please Contact Us for more information.
Specifications
C of A & Other Certificates
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
Sample C of A
This is a sample C of A and may not represent a recently manufactured lot of the product.
A sample C of A for this product is not available at this time.
Analytical Charts
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
The requested analytical chart is not available. Sorry for the inconvenience.