NAM [=N-(9-Acridinyl)maleimide] [for HPLC Labeling]

The compound 1 is an HPLC fluorescence labeling reagent, and can easily react with a mercapto group at room temperature. The resultant sulfide is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection analysis at the excitation and emission wavelengths of 355 nm and 465 nm, respectively.

Application example:
[Thiols] ^1~5)
0.4 mL of 30% NaOH solution and 1 mL of 0.2 M boric acid buffer solution (pH 8.8) are added to 2 mL of 1 mM sample solution in water. To this solution, 0.5 mL of 10 mM labeling reagent 1 / acetone solution is added and shaken, and reacted at room temperature for 30 min to use it as a HPLC sample.

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )