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Published TCIMAIL newest issue No.198

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CAS RN: 129-06-6 | Product Number: W0005

Warfarin Sodium (contains Isopropyl Alcohol)

Purity: >98.0%(HPLC)

Synonyms:

3-(α-Acetonylbenzyl)-4-hydroxycoumarin Sodiuim Salt (contains Isopropyl Alcohol)
4-Hydroxy-3-(3-oxo-1-phenylbutyl)coumarin Sodium Salt (contains Isopropyl Alcohol)

Product Documents:

Size	Unit Price	Belgium	Japan*	Quantity
5G	€36.00	Contact Us	≥40
25G	€135.00	2	≥40

*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).

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General Information plus

Product Number	W0005
Purity / Analysis Method	>98.0%(HPLC)
Molecular Formula / Molecular Weight	C₁₉H₁₅NaO₄ = 330.31
Physical State (20 deg.C)	Solid
Storage Temperature	Room Temperature (Recommended in a cool and dark place, <15°C)
Store Under Inert Gas	Store under inert gas
Condition to Avoid	Air Sensitive
CAS RN	129-06-6
Related CAS RN	81-81-2
Reaxys Registry Number	5469050
PubChem Substance ID	87560363
Merck Index (14)	10038
MDL Number	MFCD00083223

Specifications & Properties plus

Safety & Regulations plus

GHS

Pictogram
Signal Word	Danger
Hazard Statements	H300 : Fatal if swallowed. H360 : May damage fertility or the unborn child. H370 : Causes damage to organs. H372 : Causes damage to organs through prolonged or repeated exposure. H373 : May cause damage to organs through prolonged or repeated exposure. H412 : Harmful to aquatic life with long lasting effects.
Precautionary Statements	P260 : Do not breathe dust. P201 : Obtain special instructions before use. P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection. P308 + P311 : IF exposed or concerned: Call a POISON CENTER/doctor. P301 + P310 + P330 : IF SWALLOWED: Immediately call a POISON CENTER/doctor. Rinse mouth.

Related Laws:

EC Number	204-929-4
RTECS#	GN4725000

Transport Information:

UN Number	UN1544
Class	6.1
Packing Group	II
HS Number	2932209090

Applications & Literature plus

Application

Warfarin Sodium (contains Isopropyl Alcohol) Binding of Warfarin to Human Serum Albumin

Warfarin is known to have affinity for Human Serum Albumin (HSA) and to bind (interact) to drug binding site I on HSA. Those were confirmed using our warfarin sodium with Surface Plasmon Resonance (SPR) and a method using fluorescent probes.

【SPR】
Dose responses of warfarin to HSA were confirmed by SPR. Biacore, as a SPR biosensor, was used for the assay, according to the user’s guide of the instrument.

W0005

＜Assay condition＞
Sensor Chip: Series S Sensor Chip CM5, Immobilization: HSA (Amine Coupling method), Buffer : 5%DMSO in PBS.

＜Result＞
“Square wave” sensorgrams were exhibited at each concentration, and concentration dependent binding of warfarin to HSA was confirmed.

【Method using fluorescent probes】
The drug biding site of warfarin was confirmed using fluorescent probes which bind to drug binding site on HSA. Dansylamide (DNSA) [D5405] was used as fluorescent probe for site I, and dansylglycine (DNSG) [D5406], BD140 [D4898] were used as fluorescent probes for site II, and then bindings to site I and site II were confirmed.

W0005

＜Assay condition＞
Buffer: 1 % DMSO in phosphate buffer (pH 7.2 - 7.5); HSA: 5 µM (DNSA), 20 µM (DNSG, BD140) (50 µL/well) (Fatty acid free HSA is recommended.); Warfarin: each concentration (50 µL/well); DNSA: 80 µM, DNSG: 20 µM, BD140: 20 µM (50 µL/well); Incubation: 20-25 °C for 30 min; Measurement: plate-reader with excitation = 365 nm and emission = 480 nm (DNSA, DNSG), with excitation = 365 nm and emission = 585 nm (BD140).

＜Result＞
As shown in upper diagram, inhibition against binding of dansylamide which is fluorescent probe for site I by warfarin was confirmed. And also, little or extremely weak inhibition against binding of dansylglycine and BD140 which are fluorescent probes for site II was confirmed.

In these ways, our warfarin can be used for study of interaction with HSA. Also, DNSA [D5405], DNSG [D5406] and BD140 [D4898] can be used for study of drug binding site on HSA.

References

Fluorescent Dye Cocktail for Multiplex Drug￢Site Mapping on Human Serum Albumin
- J. C. Er, M. Vendrell, M. K. Tang, D. Zhai, Y. T. Chang, ACS Comb. Sci. 2013, 15, 452.
High-resolution and high-throughput protocols for measuring drug/human serum albumin interactions using BIACORE
- R. L. Rich, Y. S. Day, T. A. Morton , D. G. Myszka, Anal Biochem. 2001, 296, 197.
Biosensor Analysis of the Interaction between Immobilized Human Serum Albumin and Drug Compounds for Prediction of Human Serum Albumin Binding Levels
- Å. Frostell-Karlsson, A. Remaeus, H. Roos, K. Andersson, P. Borg, M. Hämäläinen, R. Karlsson, J. Med. Chem. 2000, 43, 1986.
Characterizing a drug's primary binding site on albumin
- Y. S. Day, D. G. Myszka, J. Pharm. Sci. 2003, 92, 333.
Structural basis of the drug-binding specificity of human serum albumin
- J. Ghuman, P. A. Zunszain,I. Petitpas, A. A. Bhattacharya, M. Otagiri, S. Curry, J. Mol. Biol. 2005, 353, 38.

Application

Reviews of Pharmacology of Warfarin

References

Comparative pharmacokinetics of vitamin K antagonists: warfarin, phenprocoumon and acenocoumarol
- M. Ufer, Clin. Pharmacokinet. 2005, 44, 1227.
Systematic overview of warfarin and its drug and food interactions
- A. M. Holbrook, J. A. Pereira, R. Labiris, H. McDonald, J. D. Douketis, M. Crowther, P. S. Wells, Arch. Intern. Med. 2005, 165, 1095.
Pharmacogenetics of warfarin elimination and its clinical implications
- H. Takahashi, H. Echizen, Clin. Pharmacokinet. 2001, 40, 587.
Pharmacogenetics of warfarin: current status and future challenges
- M. Wadelius, M. Pirmohamed, Pharmacogenomics J. 2007, 7, 99.
Pharmacogenetics of warfarin: regulatory, scientific, and clinical issues
- B. F. Gage, L. J. Lesko, J. Thromb. Thrombolysis. 2008, 25, 45.

PubMed Literature

Articles/Brochures

Fluorescent Probes for the Characterization of Two Specific Drug Binding Sites on HSA

Polyphenols

TCIMAIL

[Product Highlights] Fluorescent Probe for Human Serum Albumin (HSA)-Drug Binding Assay

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Product Categories

Life Science

Chemicals by Class

6-Membered Heterocyclic Compounds [Chemical Structural Class]

Pyrans [Chemical Structural Class]

Coumarins [Chemical Structural Class]

Life Science

Chemicals by Class

6-Membered Heterocyclic Compounds [Chemical Structural Class]

Pyrans [Chemical Structural Class]

Coumarins [Chemical Structural Class]

Appearance	White to Almost white powder to crystal
Purity(HPLC)	min. 98.0 area%
Purity(Nonaqueous Titration)	min. 91.0 %
2-Propanol	7.0 to 9.0 %

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CAS RN: 129-06-6 | Product Number: W0005

Warfarin Sodium (contains Isopropyl Alcohol)

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Safety Data Sheet (SDS)

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CAS RN: 129-06-6 | Product Number: W0005

Warfarin Sodium (contains Isopropyl Alcohol)

References

References

Articles/Brochures

Safety Data Sheet (SDS)

Specifications

C of A & Other Certificates

Sample C of A

Analytical Charts

Other Documents

Product Categories

Life Science

Chemicals by Class

Life Science

Chemicals by Class