Published TCIMAIL newest issue No.196
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CAS RN: | Product Number: G0597
Goat Anti-Rabbit IgG Biotin Conjugate
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| Size | Unit Price | Belgium | Japan* | Quantity |
|---|---|---|---|---|
| 1VIAL |
€99.00
|
Contact Us | 21 |
|
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
Supplemental Product Information:
This product is the successor to Goat Anti-Rabbit IgG Biotin Conjugate (Preservative : 0.05% NaN3, Stabilizer : 1% BSA) (0.1mg/vial) (Product Number: G0389). Please use this product if you have used G0389.
Product Information
Volume/Quantity : 0.1 mgProduct Form : Purified goat polyclonal antibody/IgG, biotin conjugated
Antibody : Antiserum was prepared by injection of purified whole rabbit
Preparation : IgG into healthy goat without clinical history of infectious disease.
Conjugation : Affinity purified antibody was labeled with a biotin.
Buffer : Phosphate buffered-saline(-) (PBS(-))
Preservative : 0.05% Sodium azide
Immunogen : Whole rabbit IgG
Specificity : Rabbit IgG
Application
ELISA (1:5000). Western blotting (1:5000). Immunofluorescence (1:200).
Storage
Store at −20 °C to −80 °C (Preferably at −80 °C). It is not recommended storing at 4 °C. Avoid repeated freezing and thawing.
Related Products
G0386 Goat Anti-Mouse IgGG0387 Goat Anti-Mouse IgG Biotin Conjugate
G0407 Goat Anti-Mouse IgG HRP Conjugate
G0406 Goat Anti-Mouse IgG FITC Conjugate
G0505 Goat Anti-Mouse IgG DTBTA-Eu3+ Conjugate
G0569 Goat Anti-Mouse IgG R-PE Conjugate
G0408 Goat Anti-Mouse IgM
G0432 Goat Anti-Mouse IgM Biotin Conjugate
G0417 Goat Anti-Mouse IgM HRP Conjugate
G0453 Goat Anti-Mouse IgM FITC Conjugate
G0388 Goat Anti-Rabbit IgG
G0418 Goat Anti-Rabbit IgG HRP Conjugate
G0452 Goat Anti-Rabbit IgG FITC Conjugate
G0506 Goat Anti-Rabbit IgG DTBTA-Eu3+ Conjugate
G0577 Goat Anti-Rabbit IgG R-PE Conjugate
S0951 Streptavidin from Streptomyces avidinii
S0972 Streptavidin HRP Conjugate
S0966 Streptavidin FITC Conjugate
S0993 Streptavidin DTBTA-Eu3+ Conjugate
T3885 Streptavidin R-PE Conjugate
T3531 Streptavidin Maleimide Conjugate
| Product Number | G0597 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Frozen (-80°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Almost colorless clear liquid |
| Titer(ELISA) | to pass test |
Properties (reference)
GHS
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Transport Information:
| HS Number | 2933399990 |
Application
Western Blotting
- Detection of Tubulin
- Samples (Reduced HeLa Whole Cell Lysate)
- Lane 1 : 1.00 µg
- Lane 2 : 0.50 µg
- Lane 3 : 0.10 µg
- Lane 4 : 0.05 µg
Post-transfer PVDF membrane was blocked using BSA/TBST and incubated in sequence with diluted primary antibody (Rabbit Anti α-Tubulin IgG), Goat Anti-Rabbit IgG Biotin Conjugate [G0597] 0.2 µg/mL, and Streptavidin HRP Conjugate [S0972] 0.2 µg/mL, followed by visualization with 3,3'-diaminobenzidine (DAB).
Application
Immunofluorescence Staining
The HeLa cells were fixed with 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100. Then, the specimen was blocked in BSA/PBS blocking buffer. Subsequently, the specimen was incubated with properly diluted primary antibody (Rabbit Anti α-Tubulin IgG). And, after washing, was further incubated with biotin-conjugated secondary antibody (Goat Anti-Rabbit IgG Biotin Conjugate [G0597] 5 µg/mL). Finally, it was rinsed with PBS and stained with Streptavidin R-PE Conjugate [T3885] 5 µg/mL (shown in red) and Bisbenzimide H 33258 (1mg/mL in Water) [B6236] 1 µg/mL (shown in blue).
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Specifications
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